Recirculating Chillers
Sigma Aldrich Fine Chemicals Biosciences TRI ReagentR LS for proces
TRI Reagent LS is a quick and convenient reagent for use in the simultaneous isolation ofRNA DNA and proteinfrom liquid samples of human animal plant yeast bacterial and viral origin. A convenient single-step liquid phase separation results in the simultaneous isolation of RNA DNA and protein. This procedure is an adaptation of the singlestep method reported by Chomczynski and Sacchi for total RNA isolation and permits fast and efficient processing of liquid samples. TRI Reagent LS performs well with large or small sample volumes and many samples can be simultaneously extracted.TRI Reagent LS is a mixture of guanidine thiocyanate and phenol in a monophase SOLUTIONon. When a biological sample is homogenized or lysed with it and chloroform or 1-bromo-3-chloropropane is added the mixture separates into 3 phases an aqueous phase containing RNA the interphase containing DNA and an organic phase containing proteins. Each component can then be isolated after separating the phas
Sigma Aldrich Fine Chemicals Biosciences Asialofetuin fetal ca250MG
Asialofetuin is a glycoprotein with three asparagine-linked triantennary complex carbohydrate chains and terminal N-acetylgalactosamine residues.
Sigma Aldrich Fine Chemicals Biosciences ReadyShieldR Protease and
Protease and Phosphatase Inhibitor Cocktail has been optimized and tested for mammalian cellular and tissue extracts. It contains inhibitors with a broad specificity for serine cysteine acid proteases aminopeptidases L-isozymes of alkaline phosphatases and serine/ threonine protein phosphatases. Protease and Phosphatase Inhibitor Cocktail has been tested on extracts from various animal tissues such as human placenta and bovine liver and on extracts of A431 CHO and U937 cells. The cocktail is supplied as a ready-to-use SOLUTIONon using a proprietary non-freezing formulation. ReadyShield(R) PPC2020 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail PPC1010
Sigma Aldrich Fine Chemicals Biosciences PolyDL lactide co glycolid5G
Poly(D L-lactide-co-glycolide) is a biodegradable and biocompatible polymer which is used for drug delivery. It is used to treat rheumatoid arthritis ulcerative colitis Crohn disease inflammatory lung diseases and ophthalmic inflammatory disorders. Poly(D L-lactide-co-glycolide) functions as a potential cancer therapeutic target.
Sigma Aldrich Fine Chemicals Biosciences CelLytcTM B Cell Lyss Re250ML
CelLytcTM B Cell Lyss Re250ML
Sigma Aldrich Fine Chemicals Biosciences fibronectin pure
fibronectin is responsible for the cellular interactions with the extracellular matrix. It is involved in cell adhesion migration growth and differentiation. It is a soluble component of plasma and other body fluids. In addition it is also part of the insoluble extracellular matrix.
Sigma Aldrich Fine Chemicals Biosciences PhotoHA-LAP Methacrylated
3D cell culture including bioprinting allows for the creation of more physiological cell models by allowing cells to simultaneously interact with integrins on all cell surfaces resulting in the activation of specific signaling pathways not activated in traditional 2D cell culture methods. Hydrogels are water swollen polymers that allow for the culture of cells in 3-dimensions and can have profound effects on cellular development differentiation migration and function. New areas of tissue engineering such as 3D bioprinting have utilized UV photocrosslinked methacrylated hydrogel biomaterials (PEGMA GelMA HAMA and ColMA etc.) to encapsulate cells to make printable bioinks. Hyaluronic acid is the most abundant glycosaminoglycan in the body being an important component of several tissues throughout the body. While it is abundant in extracellular matrices hyaluronan also contributes to tissue hydrodynamics movement and proliferation of cells and participates in a number of cell
Sigma Aldrich Fine Chemicals Biosciences KiCqStartR SYBRR Green qPC
KiCqStart SYBR Green qPCR ReadyMix is a 2X concentrated ready-to-use reaction cocktail that contains all components except primers and template for real-time quantitative PCR (qPCR) This unique combination of proprietary buffer stabilizers and Hot-Start Taq DNA polymerase delivers maximum PCR efficiency sensitivity specificity and robust fluorescent signal using fast or conventional cycling protocols with SYBR Green qPCR.Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step. The optimized formulation includes SYBR Green I dye an antibody-mediated Hot-Start Taq DNA polymerase dNPTs MgCl2 and proprietary buffers and stabilizers.
Sigma Aldrich Fine Chemicals Biosciences Staurosporine Staurosporin
Suitable for use as a potent inhibitor of PKC and other protein kinases in vitro and in cultured cells.
Sigma Aldrich Fine Chemicals Biosciences L alpha Phosphatdyl L ser100MG
L alpha Phosphatdyl L ser100MG
Sigma Aldrich Fine Chemicals Biosciences CelLyticTM Express500ML
CelLytic(TM) Express is a non-denaturing and highly efficient protein extraction formulation for in-culture bacterial cell lysis. This proprietary powder formulation extracts 2-3 times more protein than conventional methods such as sonication. In addition the method is fast and requires less sample manipulations reducing proteolytic degradation and preserving protein activity. Unlike other in-culture lysis products CelLytic(TM) Express is a complete formulation including lysozyme and endonuclease. The resulting lysate is completely clear of cellular debris and is immediately ready for affinity purification. CelLytic(TM) Express unique powder formulation adds minimal volume to the final lysate. It provides a cell lysis method with defined volume and time parameters making it easier to validate and more convenient for production scale protocols.
Sigma Aldrich Fine Chemicals Biosciences DuranR laboratory bottles
Reusable bottles and caps for the storage of reagents chemicals SOLUTIONons tissue culture media etc. Made of Duran borosilicate glass Type 3.3 for maximum chemical and thermal shock resistance.